![]() The crab is a moderate, asymmetrical hyper/hypo-osmoregulator but is a strong, asymmetrical hyper/hypo-chloride regulator. Further, we investigate posterior gill (Na +, K +)-ATPase kinetics, α-subunit immunolocalization and its mRNA and protein expression (15, 30 and 40 or 45 ‰S). We evaluate osmotic and chloride regulation in Callinectes danae after 10 days acclimation to a wide salinity range (5–50 ‰S), accompanying alterations in hemolymph osmolality and during hypo- (15 ‰S) or hyper- (45 ‰S) osmotic challenge. The gill (Na +, K +)-ATPase underpins such hyper/hypo-regulatory mechanisms. Salt tolerance reflects ecophysiological adaptation, and the wide-ranging distribution of the Brachyura mirrors their ability to adjust body fluid concentrations. ![]()
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